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KMID : 1145120200180030437
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Asian Journal of Beauty and Cosmetology
2020 Volume.18 No. 3 p.437 ~ p.445
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Evaluation Biological Activities of Diphylleia grayi Extract as a Natural Cosmetic Ingredient
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Lee Gi-Bok
Yeom A-Reum
Park Chang-Min
Joung Min-Seok
Lee Gi-Yong
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Abstract
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Purpose : In this study, we investigated the biological activities of Diphylleia grayi extract as a natural cosmetic ingredient.
Methods : The antioxidant activities of Diphylleia grayi extract was measured through 2,2-diphenyl-1-(2,4,6-trinitrophenyl)-hydrazinyl (DPPH), 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) scavenging assay and Fluorescence recovery after photobleaching (FRAP) assay. We further investigated whether Diphylleia grayi extract could reduce the intracellular reactive oxygen species (ROS) using dichlorofluorescin diacetate (DCF-DA) fluorescence. To investigate the whitening effect, we conducted tyrosinase inhibition assay and L-DOPA oxidation inhibition assay. Furthermore, we performed collagenase inhibition assay to evaluate the anti-wrinkle activity of Diphylleia grayi extract.
Results : Diphylleia grayi extract showed strong antioxidant activities on DPPH, ABTS radical scavenging assay. Furthermore, Diphylleia grayi extract decreased the intracellular ROS on skin normal cells. Diphylleia grayi extract has also inhibition activities of tyrosinase, L-DOPA oxidation and collagenase.
Conclusion : Therefore, Diphylleia grayi extract was identified as a promising natural cosmetic ingredient which has antioxidant, whitening and anti-wrinkle properties.
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KEYWORD
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Diphylleia grayi, Reactive oxygen species, Antioxidant, Tyrosinase, Collagenase
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